86-3947-71　［取扱停止］CAS9 Polyclonal Antibody 60uL　E-AB-66378

特徴

• 背景：CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.

仕様

• 抗体タイプ：Polyclonal Antibody
• アプリケーション：WB,IF
• 交差性：Streptococcus pyogenes,Human
• 宿主：Rabbit
• アイソタイプ：IgG
• 抗原：Recombinant protein of streptococcus pyogenes CAS9
• 緩衝液：PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• 精製方法：Affinity purification
• 希釈倍率：WB 1:5000-1:10000 IF 1:50-1:200
• 標識：Unconjugated
• メーカーマニュアル：https://file.elabscience.com/viewpdf-389630-Elabscience-E-AB-66378.pdf
• サイズ：60uL
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