Human Colorectal Adenocarcinoma （LS 174T） cells are isolated from a Dukes type B colorectal adenocarcinoma tissue by by centrifugal force following enzymatic digestion. GFP-expressing Human Colorectal Adenocarcinoma （GFP-LS 174T） are selected from LS 174T cells after infected with lentiviruses expressing GFP with puromycin. The cells are shipped in a frozen vial with more than 5 x 105 cells/vial. The Universal Full Growth Medium are recommended to culture the cells and these cells have a minimum population doubling capacity ＆gt; 20 when cultured following the detailed protocol described below.
