Transformed from huamn B lymphocyte cell line（from 61 years male）, the Luciferase and RFP was constitutively co-expressed under EF1a promoter as two seperated proteins（not as fusion）, mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter.Cells demonstrate strong RFP fluorescent signal, and luciferase assay signal via Luc assay（Note： It is the pooled stable cell population, not originated from a single cell colony. Thus you will observe the uneven RFP signal intensity amount cells）.
