The Glucocorticoid Receptor Pathway GAL4 Reporter （Luc） – HEK293 Cell Line contains a firefly luciferase gene under the control of glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain （DBD） of GAL4 （GAL4 DBD-GR） stably integrated into HEK293 cells. This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence （UAS）. This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of DXMS and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.
