This cell line expresses a surface human GITR （glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357） and an NF-ĸB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.
