Mouse Liver Microvascular Endothelial Cells （mLivMVECs） were initially isolated from mouse liver tissues of 3-week old C57/Black6 mice and SV40-GFPmLivMVECs were selected from mLivMVECs infected with SV40 and GFP expressing lentiviruses with puromycin. Endothelial Growth Medium is recommended for the expansion of SV40-GFP-mLivMVECs. And we warranty a minimum of 30 passages of the cells if cultured following the detailed protocol described below.
