As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC （cDC） acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The Wild-type MutuDC1940 cell line is an Immortalised splenic CD8＆amp;alpha;+ subset （CD11chigh,B220-,DEC205+,CD24high,CD11b-） of conventional dendritic cells derived from the CD11c：SV40LgT transgenic mice. The MutuDC1940 cell line retains response to TLR ligands such as CpG （TLR9-L） and PolyIC （TLR3-L） and to a lesser extent LPS （TLR4-L） stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens.
