Immortalised Mouse Kupffer Cells （ImKC） are isolated from male H-2Kb-tsA58 transgenic mice which expresses the thermolabile mutant tsA58 of SV40 40 large T antigen and were sorted through identifying the F4/80 marker. The Immortalised cells expresses p53 levels with increases telomerase activity and retains the some functional features compared to the primary cells. ImKC cells can propagate without the presence of IFNy at 37°C, and are able to produce proinflammatory cytokines when induced with lipopolysaccharide （LPS）. These cells are valuable for studies in proinflammatory cytokines （TNF-α, IL-6 and IL-1β） and their roles in Kuppfer cells, in processes in liver injury, and for research in chronic diseases.
