HAdMSCs are isolated from lipoaspirate derived from white adipose tissue and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HAdMSCs are puromycin resistant HAdMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HAdMSCs is tested to ensure the cells can be passaged at least three times （i.e., approximately 9 to 10 population doublings） after thaw in complete growth media （Mesenchymal Stem Cell Growth Medium （MSCGM））, when cultured following the detailed protocol described below.
