Mouse Induced Pluripotent Stem （iPS） Cells-derived Endothelial Cells （iPS-ECs） are isolated from mouse dermal fibrolasts of pathogen-free laboratory mice. iPS-ECs are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen's' Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106 cells per ml and are delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
