Urothelial carcinoma cell line. MB49 cells are derived from C57BL/Icrf-a mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen on the second day of a long term primary culture. Transformed cells transplanted into syngeneic mice were shown to generate carcinomas . While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100％ of the cells analyzed . This abnormality is a frequent early event in human bladder cancer.
