Tumorigenecity： Yes, produces tumors in nude mice consistent with type II bronchiolar adenocarcinoma; Isoenzyme： G6PD, B;PGM1, 1;PGM3, 2;ES-D, 1;Me-2, 2;AK-1, 1;GLO-1, 2 Histopathology： carcinoma, squamous cell; Grade IV; Subculture： Remove medium, rinse with fresh 0.25％ Trypsin solution, remove trypsin and let the culture sit at room temperature （or 37℃） until the cells detach （about 10min）.Add fresh medium, aspirate and dispense into new flasks.Subculture every 6 to 8 days. Split ratio： 1： 2; Media change： 2 to 3 times per week; Reverse transcritase： Production.
