Histopathology： carcinoma, squamous cell; Pleural effusionSubculture： Remove medium, rinse with fresh 0.25％ Trypsin solution, remove trypsin and let the culture sit at room temperature （or 37℃） until the cells detach （about 10min）.Add fresh medium, aspirate and dispense into new flasks.Subculture every 6 to 8 days.Split ratio： 1： 3 to 1： 6 Media change： 2 to 3 times per weekReverse transcritase： Production＆lt;/p＆gt;.
