Tumorigenecity： Isoenzyme： Histopathology： leukemia, chemically inducedSubculture： Remove spent medium, add fresh 0.25％ Trypsin, 0.03％ EDTA solution, rinse and remove trypsin. Add fresh trypsin solution （1 to 2 ml） and let the culture sit at room temperature （or at 37℃） until the cells detach.Add fresh medium, aspirate and dispense into new flasks.Split ratio： 1： 4 to 1： 8Media change： every 2 to 3 daysReverse transcritase： Productionhistamine.
