The M2-10B4 cell line is a clone derived from bone marrow stromal cells from a （C57BL/6J X C3H/HeJ） F1 mouse.The cells express laminin and collagen 4, but do not express collagen 1 or factor 8The cells will support human and murine myelopoiesis in long term culture, and will support certain murine stromal cell dependent pre-B cell lines in vitro.Tumorigenecity： Isoenzyme： Histopathology： Subculture： Remove spent medium, add 0.25％ Trypsin solution, rinse and remove trypsin. Keep the cells at room temperature until the cells detach （about 5 to 10 minutes）. Add fresh medium, aspirate and dispense into new flasks.Split ratio： Media change： Reverse transcritase： Productionlaminin; Collagen 4.
