Human kidney adenocarcinoma. Derived from 78-year-old male. Does not produce tumours in nude mice. Tumorigenecity： No. Isoenzyme： Me-2, 1;PGM3.1-2;PGM1, 1;ES-D, 1;AK-1, 1;GLO-1, 2;G6PD, B; Histopathology： adenocarcinoma; Subculture： Remove medium, rinse with fresh 0.25％ Trypsin solution, remove trypsin and let the culture sit at room temperature （or at 37℃） until the cells detach （about 10 min）. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Split ratio： 1： 3 to 1： 4; Media change： 2 to 3 times weekly; Reverse transcritase： Production.
