Primary human immature dendritic cells （DCs） were derived from immunomagnetically selected peripheral blood （PB） monocytes. Monocytes were cultured in RPMI 1640 Medium with 10％ fetal bovine serum （FBS）, GM-CSF and IL-4 for 5 days to generate immature DCs. PB was collected using one of the following anticoagulants： acid-citrate-dextrose （ACD）, acid-citrate-dextrose solution A （ACDA）, citrate-phosphate-dextrose （CPD）, citrate-phosphate-double-dextrose （CP2D）, or citrate-phosphate-dextrose-adenine （CPDA）.
