Human Kupffer Cells are derived from human liver tissue. Cells are grown in tissue culture flasks and incubated in AcceGen＆amp;rsquo; Cell Culture Medium for 2-5 days. Prior to shipping, cells at passage 0 are detached from flasks and cryopreserved in vials. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of markers using antibodies, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified byAcceGen. Repeated freezing and thawing of cells is not recommended.
