Fibroblasts are mesenchymal cells which derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. The pulmonary arterial vesculature responds to various insults with a characteristic pattern of remodeling that includes an increase in connective tissue in the medial layer of the vessel wall. One of the pathogenetic mechanisms involved is the functional derangements of vascular fibroblasts. Their proliferative response contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. HPAAF from AcceGen Research Laboratories are isolated from human pulmonary artery. HPAAF are cryopreserved at passage one and delivered frozen. Each vial contains ＆gt;5×10^5 cells in 1 ml volume. HPAAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HPAAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAAF are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.
