The periodontal ligament is a connective tissue located between the cementum of teeth and the alveolar bone of the mandibula. It plays an integral role in the maintenance and regeneration of periodontal tissue. The cells responsible for maintaining this tissue are thought to be fibroblasts, which can be either multipotent or composed of heterogenous cell populations that differ in their function. Despite the similar morphology with gingival fibroblasts, periodontal ligament fibroblasts （PDLF） appear to display distinct functional activities in the maintenance of tissue integrity. It is known that PDLF produce osteoblast-related extracellular matrix proteins and show higher alkaline phosphatase activity compared with gingival fibroblasts. PDLF actively participate in immune and inflammatory events in periodontal diseases.HPDLF from AcceGen Research Laboratories are isolated from human periodontal tissue. HPDLF are cryopreserved at passage one and delivered frozen. Each vial contains ＆gt;5×10^5 cells in 1 ml volume. HPDLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HPDLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPDLF are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.
