Fibroblasts are mesenchymal cells with many vital functions during development and in adult organisms. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Fibroblasts are among the most accessible normal mammalian cell types and still the most amenable to culture in vitro. Gingival fibroblasts are the major constituents of gingival tissue and play a key role in their health maintenance. Human gingival fibroblasts （HGnF） express a wide surface molecular panel including mainly CD9, CD26, CD55, CD59, CD63, CD71, CD86 CD95, CD99 and CD117. They were also demonstrated to express mRNAs for protease-activated receptor-1 （PAR-1） and PAR-3.HGnF from AcceGen Research Laboratories are isolated from human gingiva. HGnF are cryopreserved at passage one and delivered frozen. Each vial contains ＆gt;5×10^5 cells in 1 ml volume. HGnF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HGnF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGnF are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.
