Mouse Liver Microvascular Endothelial cells （mLivMECs） are isolated from livers of 3-week old male C57BL/6 mice and GFP expressing mLivMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial （provided @ passage 3, with ＆gt; 5 x 105cells/vial）. mLivMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity （cells may be expanded for a maximum of 2-3 passages） in vitro, long term culture of mLivMECs is not recommended, even cultured following the detailed protocol described below）.
