mBMECs were isolated from E18 embryonic brain cortex microvessels of Balb/C mouse and transformed by transfected with SV40 middle T-antigen. GFP-mBMECs were selected puromycin resistant mBMECs from GFP-expressing lentiviral particle. The cells are shipped in frozen vials（the cells are provided @ passage 6） transfected mAECs. The cells are shipped in proliferating culture with ＆gt;90 confluence （the cells are provided @ passage 4-6）. DMEM containing 20％ FBS is recommended for cell culture.
