Form:Supplied as a lyophilized powder in PBS, pH 7.2, 100ug BSA. No preservative added. Reconstitute with sterile PBS, pH 7.4, or sterile 20mM Tris-saline (20mM Tris containing 0.15M NaCl), pH 7.4, to yield a final concentration of 0.1mg/ml. Also available Azide & BSA-free. See 350959.
Specificity:Recognizes the ~185kD c-ErbB2/c-Neu in overexpressing B104-1-1 cells. It is validated for WB, ICC, IP, and IHC with frozen sections. Stains frozen tissue sections of rat cells overexpressing the c-neu protein. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene. Does not cross-react with the EGF receptor.
Clone # USB:7.16.4
Isotype:IgG2a
Calc Applications Abbrev:IC IHC IP WB
Calc Crossreactivity:Hu Rt
Immunogen:B104-1-1 cells overexpressing rat c-ErbB2/c-Neu