Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes chicken Collagen Type II. Show no crossreactivity with Types I, III, IV, V, VI, IX, X or XI. In Western Blot, reacts with the TCA fragment of lathyritic Type II Collagen after digestion with mammalian collagenase. It does not react with pepsin-digested Type II Collagen. The epitope recognized is GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two terminal glycine residues constitute a key structural element of the epitope. The epitope is lost after digestion of lathyritic Type II Collagen with recombinant Stromelysin. Species Crossreactivity: human and mouse.
Clone # USB：0.N.225
Isotype：IgG2a,k
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Ch Hu Mo
Immunogen：Purified preparation of lathyritic Type II Collagen from embryonic chicken sternum. Cellular Localization: Catilage matrix.
