Purity:Purified by Protein G affinity chromatography from ascites
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity:Recognizes chicken Type II Collagen. Species Crossreactivity: human, mouse, salamander. Reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. Immunoblotting of CNBr peptides of collagen II shows that this reacts with CB 11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. In Western Blot (but not ELISA), this recognizes a1(II) as well as a3(XI) chain both of which have identical primary structure.
Clone # USB:0.N.226
Isotype:IgG1
Calc Applications Abbrev:IF IHC
Calc Crossreactivity:Ch Hu Mo
Immunogen:Purified type II collagen obtained from the adult chicken sternum by limited pepsinization. Epitope: Triple helix of type II collagen. Cellular Localization: Cartilage matrix