Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human Thrombospondin. Shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild crossreaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i.e. at low concentration of Ca2+. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat.
Clone # USB：3F353
Isotype：IgG1
Calc Applications Abbrev：FC IF IHC IP WB
Calc Crossreactivity：Bo Ca Eq Hu Mo Po Rt Sh
Immunogen：Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.
