Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human Thrombospondin. Shows no crossreactivity with fibronectin, fibrinogen or von Willebrand factor. It blocks TSP-mediated agglutination of fixed RBCs. It shows no effect on TSP-mediated agglutination of fixed, activated platelets. It inhibits both thrombin -and A23187-induced aggregation of washed, live (not fixed) platelets without affecting the secretion of serotonin. Inhibits adhesion of melanoma G361 cells to TSP. Blocks binding of C-terminal domain to Integrin-Associated Protein (IAP)/CD47. Species Crossreactivity: bovine, porcine, equine, canine, sheep, rat.
Clone # USB：3F352 (C6.7)
Isotype：IgG1
Calc Applications Abbrev：FC IF IP WB
Calc Crossreactivity：Bo Ca Eq Hu Po Rt Sh
Immunogen：Ca2+-replete, native purified human TSP from supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (Non-reduced). Epitope: Platelet- or cell-binding domain at the extreme C-terminus of TSP.
