Purity:Highly purified from mouse ascites by protein A chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Reacts with the mouse Connexin 47 protein. On Western blots of mouse thalamus homogenates and Connexin 47-transfected HeLa cells, it identified a band at ~47kD. Reactivity was confirmed in Western blots with mouse thalamus lysates. Mouse Connexin 47-transfected HeLa cells were used in both Immunoprecipitation and Western blot assays. This antibody was also used in immunofluorescence assays using paraformaldehyde-fixed mouse brain. The expected staining pattern is punctate labeling of the oligodendrocytes in the brain.
Clone # USB:4A11A2
Isotype:IgG1,k
Calc Applications Abbrev:IF IP WB
Calc Crossreactivity:Mo
Immunogen:Synthetic peptide derived from the C-terminal region of the mouse Connexin 47 protein.