Purity:Purified by Protein G affinity chromatography from tissue culture supernatant.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Specificity:Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Recognizes all His tagged proteins so far tested. Has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian cell lines. In Western Blot of bacterial extracts, does not crossreact with any endogenous products, though some crossreactivity may be seen with extracts of insect or mammalian cells.
Clone # USB:3H2201 (AD1.1.10)
Isotype:IgG1
Calc Applications Abbrev:FLISA IP WB
Immunogen:PAX6 transcription factor linked to 6-histidine tag (HHHHHH).