Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes human phosphorylated HSP27 (Ser78). Does not recognize the non-phosphorylated form of HSP27. An additional band was observed at ~54kD in longer exposures. Species Sequence Homology: canine, 100%; Mouse and rat based on GenPept database sequence where Ser78 is replaced with Asp.
Clone # USB:2T7
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to aa73-83 (YSRAL[pS]RQLSS-C) of human HSP27 with a C-terminus cysteine added for conjugation, conjugated to KLH.