Purity:Purified by Protein A/G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity:Recognizes human Involucrin. Epitope: codon 421-568 of human Involucrin at ~66-170kD. Species Crossreactivity: Gorilla, Owl monkey, porcine and canine. Does not react with mouse. In Western Blot of cultured human keratinocytes, 172115-AF reacts with a 120kD protein. It stains the involucrin in a variety of sizes: 170kD in MCF-7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in canine and a doublet of 105kD in porcine.
Clone # USB:SPM259
Isotype:IgG1,k
Calc Applications Abbrev:FLISA IF IHC IP WB
Calc Crossreactivity:Ca Hu Po
Immunogen:Purified involucrin from human keratinocytes. Cellular Localization: Cytoplasmic