Purity:Purified by Protein A/G affinity chromatography.
Form:Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168782.
Specificity:Recognizes human gp100. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. Reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Stains fetal and neonatal melanocytes, junctional and blue nevus cells, malignant melanoma and Angiomyolipoma (PEComa). Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Does not react with canine or rat.
Clone # USB:HMB45
Isotype:IgG1,k
Calc Applications Abbrev:E FC IF IHC
Calc Crossreactivity:Hu
Immunogen:Extract of pigmented melanoma metastases from lymph nodes. Cellular Localization: Cytoplasmic