Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. Also available without sodium azide. See C2069-47A.
Specificity:Recognizes the active form of human beta-catenin, dephosphorylated on Ser37 and Thr41. Non-specific proteins, of MW 65kD and 148kD, were also detected. Species Crossreactivity: mouse and rat. Does not crossreact with Drosophila. Species Sequence Homology: mouse and rat: 100%; porcine: 99/100aa; frog and chicken: 97/100aa; zebrafish: 91/100aa.
Clone # USB:3C196 (8E7)
Isotype:IgG1,k
Calc Applications Abbrev:FC IC IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD.