Specificity:Recognizes the cytoplasmic amino-terminal of Band 3 protein (90-100kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20kD. Digestion and aggregation results in detection with multiple molecular weight proteins. Since the epitope is not located at the erythrocyte surface, the antibody does not agglutinate red blood cells and its binding to the cell surface cannot be detected by an indirect agglutination assay. Species Crossreactivity: human. Does not crossreact with porcine, mouse, bovine, canine, Guinea pig or equine. Does not react with human fibroblast extract (nonerythroid).