Purity:Purified by Protein A and peptide affinity chromatography.
Form:Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity:Detects endogenous levels of human histone H3 only when di-methylated on Lys4. Does not crossreact with non-methylated, mono-methylated, or tri-methylated Lys4. In addition, does not crossreact with di-methylated or tri-methylated histone H3 Lys9, Lys27, Lys36 or histone H4 Lys20. Species Crossreactivity: monkey, mouse and rat.
Clone # USB:7H27(C64G9)
Isotype:IgG
Calc Applications Abbrev:ChIP IF IHC IP WB
Calc Crossreactivity:Hu Mk Mo Rt
Immunogen:Synthetic peptide corresponding to the N-terminus of human histone H3 in which Lys4 is di-methylated.