Purity：Purified by Protein G affinity chromatography from culture supernatant.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). This antibody is human-specific (no reactivity with feline, canine, bovine, sheep, goat, porcine, rabbit, equine). The epitope is located in the connecting region. Binding can be competed selectively by a peptide comprising aa121-133. Can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA. It does not interfere with the binding of any known vitronectin ligands. Also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well.
Clone # USB：HV2
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Human vitronectin purified from plasma by heparin-affinity chromatography.
