Purity:Purified by Protein A affinity chromatography
Form:Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:Recognizes human pulmonary SP-A. In Western Blot after SDS-PAGE it reacts with reduced forms of SP-A and weaker with non-reduced forms. Reacts specifically with human SP-A in immunohistochemical staining from paraffin embedded tissue samples. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. Reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.
Clone # USB:6F187
Isotype:IgG1,k
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Native SP-A purified from bronchioaveolar lavage from proteinosis patients adsorbed onto aluminum hydroxide gel.