Purity:Purified by Protein A affinity chromatography from culture supernatant.
Form:Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:Recognizes Peptide Histidine-Methionine (PHM). Binds human PHM and cross-reacts with PACAP (approximately 12%; incomplete, non-parallel inhibition). Does not cross-react with GLP-1, GLP-2, VIP or glucagon. Binds free PHM in solution. The ELISA dilution guideline of 1:2000 applies to PHM coated directly at 2ug/ml. For glutaraldehyde-linked PHM-ovalbumin (molar ratio 4:1) coated at 1ug/ml the dilution is 1:30000.