Purity:Purified by Protein A affinity chromatography from culture supernatant.
Form:Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:Recognizes denatured chicken ovalbumin (OA). Reacts with OA in denatured or modified form (surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA with OA directly coated onto the microtiter well. In Dot Blot reacts with native OA adsorbed onto nitrocellulose. In Western Blot (overnight) after SDS-PAGE, reacts with OA in both reduced and non-reduced forms. Epitope differs from O8075-34 and O8075-35 due to lack of mutual inhibition of antigen binding.
Clone # USB:6F163 (6G2)
Isotype:IgG2a,k
Calc Applications Abbrev:E WB
Calc Crossreactivity:Ch
Immunogen:Ovalbumin isolated from chicken egg white, ormaldehyde and lysine for 2 weeks at 35°C and heat denatured by incubation for 1 hr at 110°C.