Purity:Purified by Protein A affinity chromatography from culture supernatant.
Form:Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.No stabilizing proteins added.
Specificity:Recognizes Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No crossreactivity to antigens from Listeria monocytogenes or Escherichia coli. Epitope is lacking in PPD (culture filtrate, heat treated and precipitated with trichloroacetic acid). Reacts strongly in ELISA with M. tuberculosis cell culture filtrate or whole cells directly coated onto the microtiter wells (1). In Western Blot after SDS-PAGE reacts strongly with Ag85C and weakly with Ag85A and Ag85B.
Clone # USB:6F152
Isotype:IgG1,k
Calc Applications Abbrev:E WB
Immunogen:Bacterial press extract from Mycobacterium tuberculosis H37Rv.