Form:Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 30% glycerol.
Specificity:Recognizes both the steroid occupied and unoccupied forms of human ER-alpha. Detects a band of ~67kD by Western blot. Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ER-beta. Does not inhibit binding of ER to nuclei and DNA. Species Crossreactivity: mouse, rat, bovine and monkey.
Clone # USB:6D377
Isotype:IgG1
Calc Applications Abbrev:FC IC IHC IP WB
Calc Crossreactivity:Bo Hu Mk Mo Rt
Immunogen:Synthetic peptide corresponding to aa247-261 (EVGMMKGGIRKDRRG) of the DNA binding domain of ER-alpha (estrogen receptor-alpha). Species Sequence Homology: porcine, sheep, chicken and frog