Purity:Purified by thiophilic adsorption and size exclusion chromatography.
Form:Supplied as a liquid in PBS, 0.09% sodium azide, PEG, Sucrose.
Specificity:Recognizes beta-catenin phosphorylated at Tyr654 and gamma-catenin phosphorylated at Tyr654. Detects bands of ~90kD (beta-catenin) and ~70kD (gamma-catenin). Due to sequence homologies of the phospho-epitope surrounding Tyr654, there may be crossreactions with other proteins (e.g., Annexin A11 at 56kD, desmoplakin at 81kD, TNF-alpha-induced protein 2 at 73kD, ribosomal protein S6 kinase alpha 6 at 90kD). Species Crossreactivity: human, mouse and canine.
Clone # USB:6D230 (1B11)
Isotype:IgG1
Calc Applications Abbrev:E WB
Calc Crossreactivity:Ca Hu Mo
Immunogen:Synthetic peptide corresponding to a portion of beta-catenin phosphorylated at Tyr654. Epitope: phosphotyrosine 654 (VATpYAAA)