Specificity:Recognizes RNA Polymerase II.Excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200kD band of non-phosphorylated and 240kD band of phosphorylated Pol IIA. Reactivities on Cultured Cell Lines (tested so far): Drosophila chromosomes, PTK-2, HeLa, Hep2. Epitope recognized: 794DDYGPESRGFVENSYLA822. Species Crossreactivity: human, Drosophila
Clone # USB:5F159
Isotype:IgG1
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Dr Hu
Immunogen:Drosophila melanogaster RNA polymerase II, amino acid 794-822 DDYGPESRGFVENSYLA