Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:Recognizes human p53 (Tumor Suppressor Protein, Oncogene Protein). Reactivities on Cultured Hela, MCF-7 Cell Lines (tested so far). Excellent marker for wild-type and mutant forms of human p53 antigen. Epitope recognized by this antibody: 20[SDLWKLLPENNV]31. Stains positively ~60% of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.
Clone # USB:Bp53-11
Isotype:IgG2a
Calc Applications Abbrev:E IHC IP WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human p53 (transcription domain within the NH2 terminus)