Specificity:Stains specifically the nucleolus and Cajal bodies in interphase cells. Recognizes 180kD polypeptide in Xenopus (xNopp180), homolog to the rat polypeptide (Nopp140). When applied at high concentration to human cells the antibody reacts with a nucleolar component which, however, does not correspond to xNopp180. Species Crossreactivity: Xenopus laevis, Xenopus borealis, XLKE-A6 cells; negative with Pleurodelis, Triturus, Rana, chicken, rat, mouse.
Clone # USB:No-114
Isotype:IgG1
Calc Applications Abbrev:IF IHC IP WB
Calc Crossreactivity:Hu Xe
Immunogen:Nucleolar fraction prepared from Xenopus laevis oocytes.