Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:Recognizes human Desmocollin 3 at ~109 and 100kD. Localizes Dsc3 present in all living epidermal layers, in glandular duct cells, in basal matrix cells and outer root sheath of hair follicles, basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea and thymic reticulum cells. Most cultured cell lines derived from stratified squamous epithelia or squamous cell carcinomas are positive (e.g. A431, HaCatT). All simple epithelia and intercalated disks of myocardium are negative. Species Crossreactivity: Does not react with bovine or rodent.
Clone # USB:5F193
Isotype:IgG1
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to VHGAPFYFSLPNTSPEISRLWSLTKVC, a sequence present on theextracellular anchor domain of human Desmocollin.