Specificity:Recognizes human Cytokeratin 8,18. No significant reaction with mouse or rat. Recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. This clone is directed against the 52.2kD cytokeratin 8 (originally reported also to 45kD cytokeratin 18) in immunoblot. Epitope has been mapped to aa353-367 on alpha helical rod domain of cytokeratin 8.
Clone # USB:NCL-5D3
Isotype:IgG1
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu
Immunogen:Isolated from human breast cancer carcinoma cell line MCF-7.