Purity:Supernatant purified by subsequent thiophilic adsorption and size exclusion chromatography
Form:Supplied as lyophilized powder from serum-free cell culture supernatant in 30ul of 2X PBS containing PEG 3000, sucrose, 0.09% sodium azide. Reconstitute with 500ul of sterile, ddH2O to make a 0.2mg/ml solution.
Specificity:Recognizes CREB phosphorylated at Ser133 at ~46kD. May also recognize p30 and p38 proteins, which likely correspond to phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology with the phosphorylated CREB immunizing peptide. Species Crossreactivity: Human, rat, canine and mouse. Crossreactivity predicted based on sequence homology
Clone # USB:10E9
Isotype:IgG1,k
Calc Applications Abbrev:E WB
Calc Crossreactivity:Ca Hu Mo Rt
Immunogen:KLH-conjugated, synthetic phosphopeptide (REILSRRP(pS)YR) corresponding to amino acids 125-135 of human CREB. The immunizing sequence is identical in human and mouse