Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes phosphorylated Ser or Thr that is surrounded by Tyr or Phe at the -1 and +1 positions and Phe at the -4 position. It also recognizes peptides containing Lys instead of Phe at the -4 position. Does not crossreact with the non-phosphorylated PDK1 docking motifs or with other phosphorylated motifs. This antibody detects endogenous levels of phosphorylated proteins containing PDK1 docking motif, including phospho-Akt. Species Crossreactivity: human, mouse
Clone # USB：3i23(18A2)
Isotype：IgG2a
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptides corresponding to the PDK1 docking motif, followed by extensive screening and characterization of hybridoma clones. Motif: (F/K)XX(F/Y)(S*/T*)(F/Y).
